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duoset ancillary reagent kit 2  (R&D Systems)


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    R&D Systems duoset ancillary reagent kit 2
    Duoset Ancillary Reagent Kit 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/duoset+ancillary+reagent+kit+2/DuoSet+ELISA+Ancillary+Reagent+Kit+2/pm41999653-83-56-61
    Average 96 stars, based on 293 article reviews
    duoset ancillary reagent kit 2 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Effects of lipopolysaccharide on energy metabolism and immune cell activation in different microglia model systems.
    Article Snippet: Absorbance was determined at 530/10 nm with a FLUOstar OPTIMA reader (BMG Labtech, Ortenberg, Germany). .. To quantify the levels of IL-1β, IL-6, and TNF-α in the culture supernatants of BV-2 cells the DuoSet ELISA Mouse Kits together with the DuoSet Ancillary Reagent Kit 2 (all R&D Systems; Bio-Techne, Wiesbaden, Germany) were used according to manufacturer’s protocol. .. Absorbance was measured at 485/12 nm and readings at 570/10 nm were subtracted from these values to correct for background absorbance attributed to optical imperfections of the plate (FLUOstar OPTIMA reader; BMG Labtech, Ortenberg, Germany).

    Article Title: ZBP1 mediated restriction of Toxoplasma gondii in both the early acute and chronic brain stages of infection
    Article Snippet: .. Serum, peritoneal exudate fluid, and cell lysate cytokine levels were detected according to the manufacturer’s instructions using Quantikine ELISA (Mouse IL-12 p40 allele specific [R&D Systems; Cat#M1240]) and DuoSet ELISA (Mouse IFN-gamma [R&D Systems; Cat# DY485], Mouse TNF-alpha [R&D Systems; Cat#DY410-05], Mouse IL-1 alpha/IL-1F1 [R&D Systems; Cat #DY400-05], Mouse IL-18 [R&D Systems; Cat# DY7625-05]) with the DuoSet Ancillary Reagent Kit 2 (R&D Systems; Cat# DY008B). .. Parasite burden was measured by real-time polymerase chain reaction (PCR) of genomic DNA (gDNA). gDNA was isolated from mouse peritoneum exudate cells (PECs) and brain, with brain tissue first being homogenized in PBS using the Omni TH tissue homogenizer (Omni International), using the Isolate II Genomic DNA Kit (Bioline; Cat#BIO-52067).

    Article Title: Measurements of urinary biomarkers at 2 years following a lupus nephritis flare are associated with subsequent renal outcomes.
    Article Snippet: .. UBs were analysed by ELISA using DuoSet Ancillary Reagent Kit 2 (Catalogue #DY08B, R&D Systems) together with the following DuoSet antibody (ab) sets (R&D Systems): PF4 (Catalogue #DY795), sVCAM (Catalogue #DY809), adiponectin (Catalogue #DY1065), MCP- 1 (Catalogue #DY279) or CD163 (Catalogue #DY1607), following the manufacturer’s protocol. ..

    Article Title: Inflammatory transcriptomic signatures in a human cellular NMOSD model reveal upregulation of NF-κB and IL6 pathways
    Article Snippet: .. The Human IL6 DuoSet ELISA with DuoSet Ancillary Reagent Kit 2 (R&D Systems, Minneapolis, MN, USA) was performed according to the manufacturer. ..

    Article Title: ZBP1 mediated restriction of Toxoplasma gondii in both the early acute and chronic brain stages of infection.
    Article Snippet: .. Serum, peritoneal exudate fluid, and cell lysate cytokine levels were detected according to the manufacturer’s instructions using Quantikine ELISA (Mouse IL-12 p40 allele specific [R&D Systems; Cat#M1240]) and DuoSet ELISA (Mouse IFN-gamma [R&D Systems; Cat# DY485], Mouse TNF-alpha [R&D Systems; Cat#DY410-05], Mouse IL-1 alpha/IL-1F1 [R&D Systems; Cat #DY400-05], Mouse IL-18 [R&D Systems; Cat# DY7625-05]) with the DuoSet Ancillary Reagent Kit 2 (R&D Systems; Cat# DY008B). .. Parasite burden was measured by real-time polymerase chain reaction (PCR) of genomic DNA (gDNA). gDNA was isolated from mouse peritoneum exudate cells (PECs) and brain, with brain tissue first being homogenized in PBS using the Omni TH tissue homogenizer (Omni International), using the Isolate II Genomic DNA Kit (Bioline; Cat#BIO-52067).

    Article Title: Measurements of urinary biomarkers at 2 years following a lupus nephritis flare are associated with subsequent renal outcomes
    Article Snippet: .. UBs were analysed by ELISA using DuoSet Ancillary Reagent Kit 2 (Catalogue #DY08B, R&D Systems) together with the following DuoSet antibody (ab) sets (R&D Systems): PF4 (Catalogue #DY795), sVCAM (Catalogue #DY809), adiponectin (Catalogue #DY1065), MCP-1 (Catalogue #DY279) or CD163 (Catalogue #DY1607), following the manufacturer’s protocol. ..

    Article Title: Inflammatory transcriptomic signatures in a human cellular NMOSD model reveal upregulation of NF-κB and IL6 pathways.
    Article Snippet: .. The Human IL6 DuoSet ELISA with DuoSet Ancillary Reagent Kit 2 (R&D Systems, Minneapolis, MN, USA) was performed according to the manufacturer. ..

    Article Title: Quantitative Detection of Cathepsin B Activity in Neutral pH Buffers Using Gold Microelectrode Arrays: Toward Direct Multiplex Analyses of Extracellular Proteases in Human Serum
    Article Snippet: .. Carrier-free recombinant human cathepsin B (rhCB) (~60% 37 kDa proenzyme, ~40% 29 kDa mature enzyme), cathepsin B specific fluorogenic peptide substrate, human total cathepsin B DuoSet ELISA kit, and DuoSet ancillary reagent kit 2 were obtained from R&D Systems Inc. (Minneapolis, MN). .. Solutions of 1% bovine serum albumin (BSA) in 0.5X PB were prepared as 10 mg/mL from solid bovine serum albumin purchased from Tocris Bioscience (Bristol, UK).

    Recombinant:

    Article Title: Quantitative Detection of Cathepsin B Activity in Neutral pH Buffers Using Gold Microelectrode Arrays: Toward Direct Multiplex Analyses of Extracellular Proteases in Human Serum
    Article Snippet: .. Carrier-free recombinant human cathepsin B (rhCB) (~60% 37 kDa proenzyme, ~40% 29 kDa mature enzyme), cathepsin B specific fluorogenic peptide substrate, human total cathepsin B DuoSet ELISA kit, and DuoSet ancillary reagent kit 2 were obtained from R&D Systems Inc. (Minneapolis, MN). .. Solutions of 1% bovine serum albumin (BSA) in 0.5X PB were prepared as 10 mg/mL from solid bovine serum albumin purchased from Tocris Bioscience (Bristol, UK).



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